Serveur d'exploration sur le patient édenté

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Immobilization of Ag nanoparticles/FGF‐2 on a modified titanium implant surface and improved human gingival fibroblasts behavior

Identifieur interne : 004756 ( Main/Exploration ); précédent : 004755; suivant : 004757

Immobilization of Ag nanoparticles/FGF‐2 on a modified titanium implant surface and improved human gingival fibroblasts behavior

Auteurs : Qianli Ma [République populaire de Chine] ; Shenglin Mei [République populaire de Chine] ; Kun Ji [République populaire de Chine] ; Yumei Zhang [République populaire de Chine] ; Paul K. Chu [République populaire de Chine, Hong Kong]

Source :

RBID : ISTEX:593A4AEDC21806CCBABEF4C619D9B5F5DB1D9788

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English descriptors

Abstract

The objective of this study was to form a rapid and firm soft tissue sealing around dental implants that resists bacterial invasion. We present a novel approach to modify Ti surface by immobilizing Ag nanoparticles/FGF‐2 compound bioactive factors onto a titania nanotubular surface. The titanium samples were anodized to form vertically organized TiO2 nanotube arrays and Ag nanoparticles were electrodeposited onto the nanotubular surface, on which FGF‐2 was immobilized with repeated lyophilization. A uniform distribution of Ag nanoparticles/FGF‐2 was observed on the TiO2 nanotubular surface. The L929 cell line was used for cytotoxicity assessment. Human gingival fibroblasts (HGFs) were cultured on the modified surface for cytocompatibility determination. The Ag/FGF‐2 immobilized samples displayed excellent cytocompatibility, negligible cytotoxicity, and enhanced HGF functions such as cell attachment, proliferation, and ECM‐related gene expression. The Ag nanoparticles also exhibit some bioactivity. In conclusion, this modified TiO2 nanotubular surface has a large potential for use in dental implant abutment. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2011.

Url:
DOI: 10.1002/jbm.a.33111


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<term>Adhesion</term>
<term>Anodized</term>
<term>Antibacterial</term>
<term>Antimicrobial</term>
<term>Antimicrobial activities</term>
<term>Antimicrobial activity</term>
<term>Antimicrobial property</term>
<term>Article figure</term>
<term>Assay</term>
<term>Auger electron spectrum</term>
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<term>Biol</term>
<term>Biol chem</term>
<term>Biomed</term>
<term>Biomed mater</term>
<term>Biomedical materials research</term>
<term>Broblast</term>
<term>Broblast growth factor</term>
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<term>Cell adhesion</term>
<term>Cell adhesion number</term>
<term>Cell attachment</term>
<term>Cell fate</term>
<term>Cell line</term>
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<term>City university</term>
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<term>Collagen expression</term>
<term>Collagen expressions</term>
<term>Collagen type</term>
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<term>Contact angles</term>
<term>Contract grant number</term>
<term>Contract grant sponsor</term>
<term>Count number</term>
<term>Culture medium</term>
<term>Cytotoxicity</term>
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<term>Dental implant abutment</term>
<term>Dental implants</term>
<term>Different surfaces</term>
<term>Dimethyl sulfoxide</term>
<term>Early stage</term>
<term>Endothelial</term>
<term>Endothelial cells</term>
<term>Escherichia coli</term>
<term>Excellent cytocompatibility</term>
<term>Fibronectin</term>
<term>Gene expression</term>
<term>Gene expressions</term>
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<term>Higher gene expression</term>
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<term>Hong kong</term>
<term>Human bone marrow stromal cells</term>
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<term>Immobilization</term>
<term>Implant</term>
<term>Important role</term>
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<term>Mechanical damage</term>
<term>Multirings form</term>
<term>Nanoparticles</term>
<term>Nanotube</term>
<term>Nanotube dimension</term>
<term>Nanotubular</term>
<term>Nanotubular surface</term>
<term>Novel approach</term>
<term>Online</term>
<term>Online issue</term>
<term>Optical density</term>
<term>Osteoblast</term>
<term>Periodontal</term>
<term>Periodontal ligament cells</term>
<term>Periodontal tissues</term>
<term>Photoelectron spectroscopy</term>
<term>Positive control</term>
<term>Positive effect</term>
<term>Proc natl acad</term>
<term>Product size</term>
<term>Proliferation</term>
<term>Relative growth rate</term>
<term>Second ecvam task force</term>
<term>Silver nanoparticles</term>
<term>Soft tissues</term>
<term>Sterilization methods</term>
<term>Successive lyophilization</term>
<term>Surface roughness</term>
<term>Surface topography</term>
<term>Time intervals</term>
<term>Tio2</term>
<term>Tio2 nanotube array</term>
<term>Tio2 nanotube arrays</term>
<term>Tio2 nanotube walls</term>
<term>Tio2 nanotubes</term>
<term>Tio2 nanotubular surface</term>
<term>Tio2 nanotubular surfaces</term>
<term>Tissue engineering</term>
<term>Tissue healing</term>
<term>Titania</term>
<term>Titania nanotubes</term>
<term>Titania nanotubular surface</term>
<term>Titania surface</term>
<term>Titanium</term>
<term>Titanium surfaces</term>
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<term>Uniform distribution</term>
<term>Vegf</term>
<term>Vegf expression</term>
<term>Wiley periodicals</term>
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<term>Adhesion</term>
<term>Anodized</term>
<term>Antibacterial</term>
<term>Antimicrobial</term>
<term>Antimicrobial activities</term>
<term>Antimicrobial activity</term>
<term>Antimicrobial property</term>
<term>Article figure</term>
<term>Assay</term>
<term>Auger electron spectrum</term>
<term>Bacterial invasion</term>
<term>Biol</term>
<term>Biol chem</term>
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<term>Cell adhesion</term>
<term>Cell adhesion number</term>
<term>Cell attachment</term>
<term>Cell fate</term>
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<term>Cell proliferation</term>
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<term>City university</term>
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<term>Collagen expression</term>
<term>Collagen expressions</term>
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<term>Connective tissue</term>
<term>Contact angles</term>
<term>Contract grant number</term>
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<term>Count number</term>
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<term>Cytotoxicity</term>
<term>Cytotoxicity determination</term>
<term>Dental implant abutment</term>
<term>Dental implants</term>
<term>Different surfaces</term>
<term>Dimethyl sulfoxide</term>
<term>Early stage</term>
<term>Endothelial</term>
<term>Endothelial cells</term>
<term>Escherichia coli</term>
<term>Excellent cytocompatibility</term>
<term>Fibronectin</term>
<term>Gene expression</term>
<term>Gene expressions</term>
<term>Gingival</term>
<term>Gingival sulcus</term>
<term>Gingival tissue</term>
<term>Gingival tissues</term>
<term>Good cell culture practice</term>
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<term>Human bone marrow stromal cells</term>
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<term>Implant</term>
<term>Important role</term>
<term>Integrin</term>
<term>Mater</term>
<term>Mechanical damage</term>
<term>Multirings form</term>
<term>Nanoparticles</term>
<term>Nanotube</term>
<term>Nanotube dimension</term>
<term>Nanotubular</term>
<term>Nanotubular surface</term>
<term>Novel approach</term>
<term>Online</term>
<term>Online issue</term>
<term>Optical density</term>
<term>Osteoblast</term>
<term>Periodontal</term>
<term>Periodontal ligament cells</term>
<term>Periodontal tissues</term>
<term>Photoelectron spectroscopy</term>
<term>Positive control</term>
<term>Positive effect</term>
<term>Proc natl acad</term>
<term>Product size</term>
<term>Proliferation</term>
<term>Relative growth rate</term>
<term>Second ecvam task force</term>
<term>Silver nanoparticles</term>
<term>Soft tissues</term>
<term>Sterilization methods</term>
<term>Successive lyophilization</term>
<term>Surface roughness</term>
<term>Surface topography</term>
<term>Time intervals</term>
<term>Tio2</term>
<term>Tio2 nanotube array</term>
<term>Tio2 nanotube arrays</term>
<term>Tio2 nanotube walls</term>
<term>Tio2 nanotubes</term>
<term>Tio2 nanotubular surface</term>
<term>Tio2 nanotubular surfaces</term>
<term>Tissue engineering</term>
<term>Tissue healing</term>
<term>Titania</term>
<term>Titania nanotubes</term>
<term>Titania nanotubular surface</term>
<term>Titania surface</term>
<term>Titanium</term>
<term>Titanium surfaces</term>
<term>Type collagen</term>
<term>Uniform distribution</term>
<term>Vegf</term>
<term>Vegf expression</term>
<term>Wiley periodicals</term>
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<div type="abstract" xml:lang="en">The objective of this study was to form a rapid and firm soft tissue sealing around dental implants that resists bacterial invasion. We present a novel approach to modify Ti surface by immobilizing Ag nanoparticles/FGF‐2 compound bioactive factors onto a titania nanotubular surface. The titanium samples were anodized to form vertically organized TiO2 nanotube arrays and Ag nanoparticles were electrodeposited onto the nanotubular surface, on which FGF‐2 was immobilized with repeated lyophilization. A uniform distribution of Ag nanoparticles/FGF‐2 was observed on the TiO2 nanotubular surface. The L929 cell line was used for cytotoxicity assessment. Human gingival fibroblasts (HGFs) were cultured on the modified surface for cytocompatibility determination. The Ag/FGF‐2 immobilized samples displayed excellent cytocompatibility, negligible cytotoxicity, and enhanced HGF functions such as cell attachment, proliferation, and ECM‐related gene expression. The Ag nanoparticles also exhibit some bioactivity. In conclusion, this modified TiO2 nanotubular surface has a large potential for use in dental implant abutment. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2011.</div>
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